ISCID News Editor
Member # 1417
posted 20. November 2005 19:10
Dicing and slicing: The core machinery of the RNA interference pathway
Source: Science Direct
Edited by Shou-Wei Ding
Scott M. Hammond,
Received 1 August 2005; revised 25 August 2005; accepted 28 August 2005. Available online 27 September 2005.
RNA^ interference ( RNAi^) is broadly defined as a gene silencing pathway that is triggered by double-stranded RNA (dsRNA). Many variations have been described on this theme. The dsRNA trigger can be supplied exogenously, as an experimental tool, or can derive from the genome^ in the form of microRNAs. Gene silencing can be the result of nucleolytic degradation of the mRNA, or by translational suppression. At the heart of the pathway are two ribonuclease^ machines. The ribonuclease III enzyme^ Dicer initiates the RNAi pathway by generating the active short interfering RNA trigger. Silencing is effected by the RNA-induced silencing complex and its RNaseH core enzyme Argonaute. This review describes the discovery of these machines and discusses future lines of work on this amazing biochemical pathway.
Read the entire research article (pay to access) at Science Direct
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