Electrophoresis Contemporary Methods I-III. Electrofocusing
II. Immunoelectrophoresis
I . Electrofocusing is a very high resolution technique which separates biomolecules on the basis of their intrinsic charge. Used chiefly for peptides and proteins, electrofocusing is fast (one half to one third of the time required by traditional electrophoretic separations) and it has a high sample capacity capable of separating nearly 100 samples on one gel.
Analytical electrofocusing is widely used for quality control. Systems have been designed to employ pH gradients which are immobilised into the gel matrix during gel polymerization and thereby eliminating gradient drift and providing resolution of biomolecules differing as little as 0.001pH units in their isoelectric point.
II. Another special purpose electrophoresis method is based upon highly specific and sensitive antigen-antibody reactions, known as immunoelectrophoresis. It is also known a gamma globulin electrophoresis. This allows both the quantitative and qualitative analysis of even trace components in a highly complex mixture. It can be used to determine the blood levels of the five major immunoglobulins (i.e. antibodies), namely immunoglobulin M, immunoglobulin G and immunoglobulin A, immunoglobulin D and immunoglobulin E. It is usually performed after a different type of electrophoresis has indicated a rise in immunoglobulin level. With the growing importance of monoclonal antibodies in immunological techniques, immunoelectrophoresis has received wide acceptance.
Web Resources On Electrophoresis Contemporary Methods I-II
Wikipedia: Electrofocusing
Immunoelectrophoresis
Book Resources On Electrophoresis Contemporary Methods I-IIElectrofocusing and Isotachophoresis by Proceedings of the International Symposium
Immunoelectrophoresis: Theory, Methods, Identification, Interpretation by Pierre VC. Arquembourg
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