Electrophoresis Contemporary Methods III-IV
III. Isotachophoresis
IV. Electrophoretic Titration Curve Analysis
III. Isotachophoresis is a specialized form of electrophoresis in which separation is achieved based on difference in electrophoretic mobility. This represents a high resolution method for quantatitive / quality controls of ions including proteins, peptides, nucleosides, nucleotides and other types of metabolics.
Isotachophoresis also provides an effective method for environmental monotoring of hazardous agents found in the biotechnology industrial workplace, especially mutagenic or carconogenic potentials, such as aromatic hydrocarbons. Isotachophoresis offers several advantages over alternative electrophoretic and chromatographic procedures, including short analysis time and high sensitivity.
IV. Two-dimesional electrophoresis, introduced in 1976, combines polyacrylamide gel electrophoresis with isoelectric electrophoresis. The method is attractive because it can separate numbers of proteins. A more recent 2D technique is called electrophoretic titration curve analysis, whereby a pH gradient from 3 to 9 is established in a horizontal slab. A trough is then cut into the slab at a right angle to the gradient. The sample is applied to the trough and conventional electrophoresis is performed, perpendicular to the gradient to produce a classical titration curve. It provides useful information about the protein, including its stability and the binding of ligands. The data also predict behaviour of the protein in ion-exchange chromatography.
Web Resources On Electrophoresis Contemporary Methods III-IV
Isotachophoresis
Abstracts From Papers Employing Two-dimesional Electrophoresis
Book Resources On Electrophoresis Contemporary Methods III-IVIsotachophoresis: Theory, Instrumentation and Applications by Frans M. Everaerts
Electrophoresis in Practice by Reiner Westermeier
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