Electrophoresis Contemporary Methods V-VI-VII
V. Recycling Isoelectric focusing
VI. Electrophoretic transfer of 2D patterns
VII. Capillary Electrophoresis
V. Another recent technique, that of preparative electrophoresis, is recycling isoelectric focusing. This method employs the charge characteristics of various molecules in a solution. By “focusing” a mixture, molecules concentrate at their isoelectric point (the place on a pH gradient where a molecule loses its charge).
VI. High resolution, two-dimensional protein electrophoresis and the data associated with 2D methods will be strongly linked to DNA databases. To discern part of the coding DNA sequence, the key experimental technique in use by investigators is the electrophoretic transfer of 2D protein patterns to activated glass, after which the transferred spots are partially sequenced. The method allows the investigator to search the DNA database for the gene coding for a particular spot, and the gene identified may be isolated and cloned. Given a complete human sequence library, the gene for each protein resolved by 2D electrophoresis can be found.
VII. Capillary Electrophoresis is a technique which appeared in the late 80’s and provides rapid and automated analysis of small volumes of complex mixtures with excellent resolution and sensitivity. CE employs capillaries or nanotubes to facilitate and make more efficient the desired molecular separation.
Web Resources On Electrophoresis Contemporary Methods V-VI-VII
Principles of isoelectric focusing
Two-Dimensional Gel Electrophoresis
Book Resources On Electrophoresis Contemporary Methods V-VI-VII
Isoelectric Focusing by Nicholas Catsimpoolas
Two-dimensional Electrophoresis by A. Thomas Endler & Sam Hanash