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Electrophoresis Overview

Electrophoresis is the movement of a charged particle in response to an electric field when placed in that electric field. Electrophoresis, in concept, is several decades old but it remains a dominant instrumental approach in studies of protein, DNA and other biological substances. The core techniques for separating large and small fragments of DNA, for sequencing DNA, and for the analysis of complex mixtures of protein involve electrophoresis in microporous gels.

It is now technically possible to map and sequence the entire human genome and to resolve and identify all of the protein gene products. The most useful index to DNA databases will be based upon gene products, in as much as genes are usually named for the proteins they code for, once they have been described.

Rigid restrictions on temperature, current and composition of solutions are largely removed when electrophoresis is carried out in a solution stabilised with a material such as paper, cellulose acetate, starch, polyacrylamide gel, or agar. In addition, only minute amounts of material are required. There has been considerable specialisation in the design of electrophoresis equipment in recent years.


Web Resources On Electrophoresis Overview

Electrophoresis Tutorial
Electrophoresis - Introduction


Book Resources On Electrophoresis Overview

Electrophoresis: The Basics by D.M. Hawcroft
Capillary Electrophoresis: Theory and Practice by P. Camilleri

Related Topics

Pulsed Field Gel Electrophoresis

Gel Electrophoresis

Electrophoresis Contemporary Methods V-VI-VII


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