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Fusion Protein

A fusion protein is the product of joining two genes or two proteins / peptides together. Fusion proteins can happen naturally or it can be created artificially in a laboratory for research purposes. This is achieved through the creation of a fusion gene which is done through the removal of the stop codon from a DNA sequence of the first protein and then attaching the DNA sequence of the second protein in frame. The resulting DNA sequence will then be expressed by a cell as a single protein.

If it is two proteins that will be joined together then a linker or spacer peptide will also be added. This would usually make it more likely for the proteins to fold independently and behave as it should be. In cases wherein the linkers also enable protein purification, it is also vital that protein or peptide fusions are also engineered along with cleavage sites for proteases or chemical agents. These substances are what frees up the two separate proteins.

The making of fusion protein as a technique is commonly used for the identification and purification of proteins through the fusion of a GST protein, FLAG peptide or a hexa – his peptide.

Web Resources On Fusion Protein

Pro-Fusion� Line of Fusion Protein Reagents
Recombinant Proteins/Fusion Tags

Book Resources On Fusion Protein

Antibody Fusion Proteins by Chamow & Ashkenazi
Cloning, Gene Expression, and Protein Purification: Experimental Procedures and Process Rationale by Charles Hardin et al.

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