Genomic BlotBlots, in molecular biology, are methods for transferring DNA and RNA proteins onto a carrier, and then separating them out for analysis and other research. Analysis of genomic blots typically involves the comparison of blot patterns from various genetic samples. The patterns that emerge enable the researcher to compare genetic structure, locating critical similarity and divergence.
A blot carrier is generally something like a nitrocellulose PVDF or nylon membrane. Blots are often done after an initial gel electrophoresis, allowing the researcher to transfer molecules directly from the gel onto the blotting membrane; other times, it may be done by adding samples directly to the membrane.
After the genomic blot is done, DNA and RNA can be visualized by:
- Colorant staining, such as the silver staining of proteins
- If molecules are labeled radioactively before blotting, autoradiographic visualization
- Specific labeling of certain proteins or nucleic acids with antibodies or hybridization probes that bind only to specific molecules of the blot. With proper washing afterward, the enzymatic activities of these proteins can be visualized by incubating with reactives. Renderings may include either a colored deposit on the blot or a chemiluminescent reaction that registers on photographic film.
Some of the most often used blot methods include:
- Southern blot for DNA
- Northern blot for RNA
- Western blot for proteins
- Southwestern blot
- Dot blot
- Slot blot
Web Resources On Genomic Blot
Gene Mapping Using Genomic Blotting
Human Genomic Blot Hybridization
Book Resources On Genomic BlotProkaryotic Genomics by Michel Blot
The Genomics Age: How DNA Technology Is Transforming The Way We Life and Who We Are by Gina Smith
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