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Pulsed Field Gel Electrophoresis

Pulsed field gel electrophoresis is one of the first processes used by scientists that allows the sequencing of very long DNA samples. It's a technique using DNA electrophoresis, but because the electrical fields applied alternate from different angles, very large DNA fragments are allowed to move through the gel which permits efficient separation of these large fragments.

Pulsed field gel electrophoresis, like other DNA electrophoresis techniques, separate DNA fragments by size, using an electrical field to force fragments to move through a gel medium. The larger fragments take longer to move through the gel, so the electrophoresis process applied must not only use alternating fields but must also last longer.

After pulsed field gel electrophoresis, the DNA separated can be removed using Northern Blot technique, and then through various techniques dyed with fluorescent or radioactive markers that can help determine the makeup of the DNA fragment.

The gel most often used in pulsed field gel electrophoresis is agarose, as it provides less interference to long DNA segments.

Web Resources On Pulsed Field Gel Electrophoresis

Pulsed Field Electrophoresis for Separation of Large DNA
Partitioned pulsed-field gel electrophoresis

Book Resources On Pulsed Field Gel Electrophoresis

Methods in Molecular Biology by Burmeister & Ulanovsky
Pulsed Field Gel Electrophoresis: A Practical Approach by Anthony P. Monaco

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