Sequence Tagged Site
A sequence tagged site or STS is a short DNA sequence of usually 200 to 500 base pairs that usually happens once in a genome. The STS’ location and base sequence are known.
An STS can be detected by the polymerase chain reaction and it is useful for localizing and orienting the mapping and sequence data that is reported from many different laboratories. They also serve as the landmarks on the developing physical map of a genome. Sequence tagged sites that are derived from cDNAs (otherwise known as complementary DNAs) are called expressed sequence tags or EST. STS is believed to have an overwhelming advantage over other mapping landmarks that are defined via other methods. The reason for this is that the means of testing for the presence of a particular STS can be completely described as a piece of information in a database.
dbSTS, which is the STS division of GenBank, is intended to be used to facilitate the cross comparisons of STS with sequences that are found in other divisions. This is done for the purpose of correlating the map positions of anonymous sequences with the known genes.
Web Resources On Sequence Tagged Site
A Method of Producing STS markers from RAPDs
Sixty New STSs of Human Chromosome 21
Book Resources On Sequence Tagged SiteIntroduction to Computational Biology: Maps, Sequences and Genomes by Michael S. Waterman
Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins by Andreas D. Baxevanis, B. F. Francis Ouellette
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