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Transfection

Transfection is a method by which experimental DNA may be put into a cultured mammalian cell. Such experiments are usually performed using cloned DNA containing coding sequences and control regions (promoters, etc) in order to test whether the DNA will be expressed. Since the cloned DNA may have been extensively modified (for example, protein binding sites on the promoter may have been altered or removed), transfection is often used to test whether a particular modification affects the function of a gene.

Additional Entries On Transfection:

Transfection Methods
Transfection Reagents
Stable Transfection
Transient Transfection
Cell Transfection
siRNA Transfection

Web Resources On Transfection

A Molecular Biology Glossary

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